【ELISA视频】双抗体夹心法操作保姆级教学-未来窗影视流程

a preparation video will demonstrate different procedures using two types, c a s k three allies of kids alone bring allegiance to temperature for twenty minutes before use it a two check the kick components, according to the instruction to ensure that components are complete. component table. r t u ready to use at least a kids own equal detecting performance with regular alleged kids, but more optimized component and simplified operate procedure. component table of rt release the kids. a free wash buffer preparation if crystals are observed in the concentrated wash buffer, you can warm it with forty degree water bath, no exceed fifty degree, and makes it gently until that crystals have completely dissolved. at thirty million leader concentrated wash buffer into seven twenty million later to steal the water and mix well twenty five times solution, b sample collection and preparation. sample should be prepared in advanced following sample collection steps specified in the manual before use the sample of recommended. it'll be central, huge that ten thousand rpm for three to fifteen minute at four degree, then collect a supernatant for detection. see deluding standards and sample, see one central huge, the tube to let all standard be at the bottom of tube. at one, military leader, sample delusion buffer. heat the two bit tim, temperature for ten minutes and makes them thoroughly label seven e p tubes from first tube to seven, two at zero point, three million leader of the sample delusion buffer into each two. see three at zero point three male literacy, the above standard solution into first tube and makes them thoroughly transfer zero point three million later from first tube to second tube and mix them thorough transfer, zero point three million later from second tube to third tube, and makes them thorough. and so until six, two, seven tube only contains sample delusion, buffer will be used as black control. see four dialogue. the sample at a proper, delicious protest is highly recommended to get your best optimal solution ratio. d sample adding and incubation. d one, this mental feet, quiet strips and fixed it on palate. put the rest strips together with descended into a sealed foil bag, d two labeling set standard sample blank wells separately and accord them. right, it is recommended to measure each standard and sample and develop. definitely washing. wash played two times before adding standard sample and control blank glass. r t u a list of kids can directly do the adding without nothing before adding standard and sample. add one hundred microliter standard solution or sample solution into the relevant well noise at the solution at the bottom of each well without touching the sidewalk. d five incubation sealed a plague with plate seller notes seller should be sealed tightly to decrease the angel fact and perceive resulted from evaporation. incubate for ninety minutes at thirty seven degree. e preparation addition and incubation of violent labeled antibody working solution, even one centre, huge that concentrated antibody diluted following instructions of the manual preparative within one hour before experiment. e two washing, remove the played seller and wash the played twice with wash buffer. residual wash buffer can be moved by soaking and dumping e three, and by the labelled antibody at one hundred micro leader, buy it unlabeled antibody working solution into each. cover with you played sailor. r t u a, lisa kit, r t u, l e s a kid contains city to use antibody working solution, which can be added after washing the place directly. centrifugation and illusion are not required. before incubation incubate for sixty minutes at thirty seven degree. f preparation addition and incubation of sabc working solution, f one sent refused to concentrated s a b c and diluted following instructions of the manual prepared within thirty minutes before experiment. f two washing, remove the plate. sailor washed the plague with wash buffer three times and silk for one minute each time. residual wash buffer can be moved by soaking in f three at s a b c, add one hundred micro leader, s a b c working solution into each. cover with you played sailor. r t u a list of kit, r t, u, l, e c i t s contains ready to use s a b c working solution, which can be added directly after washing. the centrifugation and delusion are not required. a for incubation. encouraged for thirty minutes at thirty seven degree g staining g one before color reaction, it will librate t m b substrates for thirty minutes at thirty seven degree g two washing washed the plague with wash buffer five times and soak for one to two minutes each time. residual wash buffer can be moved by soaking g three atm b substrates. at ninety micro leader t m b substrate solution sealed a plague with. encouraged for ten to twenty minutes at thirty seven degree in dark condition, notes, the action time can be shortened or extended, according to the actual color change. no thirty minutes, and you can terminate the reaction when a parent gradient appeared. and standard wealth h stop and od measurement, h one stop. at fifty micro leaders stop solution into each while, the color will turn yellow immediately, notes the adding order of solution should be as the same as the t m b sub straight solution. h two read the odd absorbs at four hundred and fifty nanometers in a microplate for immediately. i resolved calculation using commended data analysis software to draw standard curve, substitute the sample of sovereign value into the equation and calculate the concentration.